Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
This focus of this webinar is on the utility of a synthetic dual RNA approach to apply CRISPR-Cas9 to HDR genomic engineering applications. It provides guidelines for improving CRISPR Cas9-assisted HDR. It also discusses the use of short single-stranded DNA as a donor template for small insertions as well as plasmid DNA donor templates for large insertions. Lastly, it outlines methods for characterization of HDR-generated cell lines for precise genomic engineering.
In this webinar, you will learn:
- Basics of homology-directed repair (HDR) using CRISPR-Cas9
- Selection of CRISPR RNAs (crRNAs) for HDR
- Recommendations for design of a synthetic oligo or plasmid donor template
- Application of endogenous gene tagging with GFP
- Techniques to evaluate HDR cell lines for the desired mutation
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