Validating the Sensitivity of Droplet Digital PCR™ Using HDx™ Reference Standards
Read how Horizon tested the sensitivity of digital PCR using Reference Standards
Digital PCR, labelled the third generation of PCR technology, enables the absolute quantitation of target DNA. The platform allows for the enrichment of rare target sequences, the detection of small differences in target concentration and the determination of copy number without the need for a standard curve.
In this study we show the Bio-Rad QX100™ ddPCR™ platform was able to detect mutant alleles present at frequencies as low as 0.05%. In addition the platform enabled the detection of mutant alleles present as low as 3.5% using DNA extracted from the FFPE Reference Standards.
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