Detecting the Onset and Kinetics of Apoptosis

In this application note we used three different readouts to analyze the kinetics of apoptosis in response to staurosporine and etoposide in cancer cell lines:

  • IncuCyte live cell imaging to track the appearance of a fluorescent caspase-3/7 dye
  • Multiplexing a cell viability dye with a caspase-3/7 dye in a plate reader-based assay
  • Analysing early and late apoptotic cell markers using western blotting and flow cytometry

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