Gene knockout, knock-in, and activation in human induced pluripotent stem cells
In this app note, we present the ability to modify the genome and modulate expression of genes within a human induced pluripotent stem cell line.
We demonstrate the following:
- Gene editing using Edit-R™ synthetic guide RNA and Cas9 nuclease
- Gene knockout to induce iPS cell differentiation - determined via live-cell fluorescent microscopy
- CRISPRa mediated activation of gene transcription
- HDR mediated knock-in of EGFP to create a fluorescently tagged protein