App note download: Gene manipulation in iPSCs

Gene knockout, knock-in, and activation in human induced pluripotent stem cells

In this app note, we present the ability to modify the genome and modulate expression of genes within a human induced pluripotent stem cell line.

We demonstrate the following:

Gene editing using Edit-R™ synthetic guide RNA and Cas9 nuclease
Gene knockout to induce iPS cell differentiation - determined via live-cell fluorescent microscopy
CRISPRa mediated activation of gene transcription
HDR mediated knock-in of EGFP to create a fluorescently tagged protein

Fill out the form below to access the app note.

Email address*

Prefix

First name*

Last Name

Company*

Job Title or Function

Country

Zip or Postal Code*

Yes, I agree to the terms and conditions and privacy policy.

Please let us know if you would like us to contact you by selecting one of the options below:

Yes, I would like to hear about offers, products and services.

No, thanks, I don’t want to know about offers, products and services.