App note download: Gene manipulation in iPSCs

Gene knockout, knock-in, and activation in human induced pluripotent stem cells

In this app note, we present the ability to modify the genome and modulate expression of genes within a human induced pluripotent stem cell line.  
We demonstrate the following:

  • Gene editing using Edit-R™ synthetic guide RNA and Cas9 nuclease
  • Gene knockout to induce iPS cell differentiation - determined via live-cell fluorescent microscopy
  • CRISPRa mediated activation of gene transcription
  • HDR mediated knock-in of EGFP to create a fluorescently tagged protein
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